His- Ade+ mutants

[Alex Grant]

Hi Yeasters

Can anybody give me some advice?  I am conducting a mutation rate experiment using S. cerevisiae.  My strains are Ade+ His4- and I am looking for prototrophs on a His- plate.  Strangely a good number of my prototrophs appear red like an Ade2- mutant.  I have searched the literature and I can't find any evidence of linkage (genetic or biochemical) between Ade2 and His4.  Any suggestions?

thanks

Alex Grant
UBC Zoology Dept.




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