genomic DNA preparation

Katja Ludin katja.ludin at izb.unibe.ch
Thu Aug 22 10:30:10 EST 2002


Hi all!

I am wondering what one sees, loading mildly prepared (spheroplast
protocol, not glass beads!) genomic DNA from S.pombe on a 1% agarose gel.
We see a more or less clean band at  around 25 kb. Should't it be a rather
high molecular DNA smear? Does anybody know a milder preparation?
Any suggestions?

Thanks,
Katja

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