S. cerevisiae cDNA library expressed in E.coli

Martin Hirst hirst at interomex.com
Tue Aug 27 19:33:48 EST 2002


On 8/23/02 5:07 AM, "Horst Irlbacher" <irlbach at molgen.mpg.de> wrote:

> Hello,
> I´m trying to set up a "south-western screen" to find S.cerevisiae proteins
> that bind to my DNA sequence of interest.
> For this reason I need to have a yeast cDNA library which is expressed in
> E.coli ( this E.coli lawn is hybridized with my labelled oligo, spots of
> specific hybridisation within the lawn should contain candidate proteins)
> To my knowledge, there is no such library commercially available. Does
> anybody a suitable library?
> We will gladly defray shipping costs. Thank you in
> advance....
> 
> Horst Irlbacher
> 
> 

If you are looking for yeast proteins which bind a specific DNA sequence why
don't you set up a one hybrid screen instead?

Martin







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