hirst at interomex.com
Mon Dec 9 22:42:04 EST 2002
On 12/7/02 13:57, "Rahima.Ziane at crhl.ulaval.ca"
<Rahima.Ziane at crhl.ulaval.ca> wrote:
Have you tried transforming the bait plasmid first, making those cells
competent and then transforming the prey plasmid? Generally it is less
efficient to transform multiple plasmids.
> Iam a PhD student and I used the proquest two hybrid method in my
> research work. Actually, I have a problem to obtain colonnies when I
> cotransform my competent cells (MaV203) with my bait plasmid and an empty
> library plasmid (in the following DNA concentration: 1ug till 100 n for
> each plasmid)
> In other hand, I obtain colonnies when I transform my cells just with an
> empty library plasmid. So, I used several dilutions of my bait plasmid
> (1000 ng till 1 ug) but the results stay the same, no colonnies.
> I will be so glad if you can help me with some advices.
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca
More information about the Yeast