lysis for cell fractionation

Peter Mastrangelo peter.mastrangelo at utoronto.ca
Thu Dec 19 17:15:09 EST 2002


Dear Yeasters,

What is the best technique for preparing yeast lysates for cell
fractionation.  Ideally, I'm looking for a technique that is gentle and
maintains the integrety of the different cellular compartments without
being too expensive (such as preparing extracts from spheroplasts made with
Zymolase).

Any suggestions would be appreciated,


Peter
Peter Mastrangelo, Ph. D.,
Centre for Research in Neurodegenerative Disease,
Tanz Neuroscience Bldg.,
University of Toronto,
6 Queen's Park Cres. West,
Toronto, Ontario, Canada.
M5S 3H2

e-mail: peter.mastrangelo at utoronto.ca
tele: (416)978-3408/(416)978-8908/(416)978-8975
FAX: (416)978-1878
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