lysis for cell fractionation

Peter Mastrangelo peter.mastrangelo at
Thu Dec 19 17:15:09 EST 2002

Dear Yeasters,

What is the best technique for preparing yeast lysates for cell
fractionation.  Ideally, I'm looking for a technique that is gentle and
maintains the integrety of the different cellular compartments without
being too expensive (such as preparing extracts from spheroplasts made with

Any suggestions would be appreciated,

Peter Mastrangelo, Ph. D.,
Centre for Research in Neurodegenerative Disease,
Tanz Neuroscience Bldg.,
University of Toronto,
6 Queen's Park Cres. West,
Toronto, Ontario, Canada.
M5S 3H2

e-mail: peter.mastrangelo at
tele: (416)978-3408/(416)978-8908/(416)978-8975
FAX: (416)978-1878

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