Pombe transformations

Jo Evans joanne.evans at wolfson.oxford.ac.uk
Tue Feb 5 10:22:04 EST 2002


Hi

I'm looking for a high efficiency transformation method for use with the pombe mutant spc1/sty1.

I have tried electroporation, but am aware from previous experience that the transformation efficiency in this strain is around 10% against wild type.

I have also tried lithium acetate, but the 42 degree heat shock tends to kill spc1!!

Does anyone have any ideas or novel transformation protocols?

Kind regards

Joanne


Joanne Evans
Chair of the Social and Cultural Committee
Wolfson College
Oxford
OX2 6UD

Cell Cycle/DNA Damage Research Groups
Radiation and Genome Stability Unit
MRC Harwell
Nr Didcot
Oxon 
OX11 0RD




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