Transformations

Jo Evans joanne.evans at wolfson.oxford.ac.uk
Wed Feb 6 13:35:26 EST 2002


Hi!
Does anyone know of a high efficiency pombe transformation method that doesn't have a heat shock element to it?
I usually use the lithium acetate method if I'm after a large number of colonies, but I have a ts strain that won't let me use this method! (electroporation doesn't give me the numbers I need).
Any ideas will be VERY greatly received.

Joanne Evans




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