Plasmid Copy Number Determination.

Jon Swaffield jon_swaffield at
Mon Jan 14 11:14:16 EST 2002

Does anyone have experience in the determination of plasmid copy number in
Saccharomyces?  I was thinking of using quantitative PCR and/or Southerns
and am sure could I derive a protocol, but would greatly appreciate some
pointers and practical advice first.

Thank you

Jonathan C. Swaffield,
Assistant Professor,
Dept. of Genetics,
North Carolina State University,
3513 Gardner Hall,
Raleigh, NC 27695-7614

Tel.       919-515-5733
Fax.     919 515-3355
Email:  jon_swaffield at

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