How to DAPI stain live S. pombe cell?

Wen Song wsong at pgec.ars.usda.gov
Tue Jan 15 12:58:04 EST 2002


Dear all,

I am new in studying S. pombe. I recently have problem to stain live S.
pombe with DAPI. Since I am using GFP fusion protein to study nuclear
localization. I need to localize the nuclear to use as an important
reference. I can stain fixed (heat or methnol)  pombe with DAPI without
problem, but my GFP fusion did not work well in fixed cell. Please help!

Wen Song
wsong at pgec.ars.usda.gov


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