DAPI staining pombe

SLF notmyaddress at hotmail.com
Wed Jan 16 11:00:20 EST 2002


>  Dear all,
>
> I am new in studying S. pombe. I recently have problem to stain live S.
> pombe with DAPI. Since I am using GFP fusion protein to study nuclear
> localization. I need to localize the nuclear to use as an important
> reference. I can stain fixed (heat or methnol) pombe with DAPI without
> problem, but my GFP fusion did not work well in fixed cell. Please help!
>
> Wen Song
> wsong at pgec.ars.usda.gov

Live DAPI staining is less efficient but works.  You can use the methods on our
pombe technology web page,
http://pingu.salk.edu/~forsburg/plasmids.html

This and other Frequently Asked Pombe Questions are covered on the pombe FAQ:
http://pingu.salk.edu/~forsburg/FAQ.html



--
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The Salk Institute, La Jolla CA
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