lin at yahoo.com
Thu Jan 17 11:46:49 EST 2002
On 16 Jan 2002 04:39:33 -0000, Michelle Bausch
<BAUSCH at rsbs.anu.edu.au> wrote:
>I have just received Lyticase from Sigma, as I wish to perform tetrad
>analysis of S. cerevisiae spores. I cannot seem to find any information on
>what buffer I should resuspend it in, and what temperature to store the
>stock solution in etc...
I recommend to prepare Lyticase freshly each time as its activity
is remarkably reduced after storage.
The dry powder itself, however, is stable for months at -20.
You can use TE to dissolve Lyticase (brownish color), works fine.
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca
More information about the Yeast