PCR from yeast collonies
s_clark at genesys.demon.co.uk
Thu Jan 24 12:17:55 EST 2002
I need to screen for a plasmid I've transformed into yeast. Can I simply
pick a collonie into water and heat at 100 degrees for 5 minutes as I
would for E.coli or do I need to do more to get the DNA out (ie.
detergent/SDS or something else).
If somebody has a quick and easy way to do this I would be most
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