Transposon mutagenesis

akabir anaul_kabir at urmc.rochester.edu
Sun Jan 27 19:21:21 EST 2002


Dear Everybody,

For transposon mediated gene disruption, we generally make the cassette in 
vitro and then linearize it and transform into yeast which land in the 
yeast genome and inserts by homologous recombination. To avoid that 
procedure, I am thinking of using Tn7 or Tn3 transposon to transform into 
yeast directly and to disrupt a gene. Does anybody have any idea about it?
    Please email me if you have some information.



Sincerely,
Kabir

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