of promotor sequence

Alex Brands abbrands at artsci.wustl.edu
Wed Jul 3 11:47:58 EST 2002


On 2 Jul 2002, [gb2312] shude yang wrote:

> Hello,my friend:
>
> This is the second time for me to ask for the same
> help: Who can tell me the promotor sequence of
> TRP1,URA3,LEU2 marker gene of yeast-E.coli shuttle
> vectors? I hope someone can help me.

You can find the complete sequence of the vectors at:

http://www.ncbi.nlm.nih.gov/

Search "Nucleotide" for the names of the vectors you are interested in.
Here are the accession numbers for three vectors:

vector   marker      accession number
------   ------      ----------------
pRS304   TRP1        U03440
pRS316   URA3        U03442
pRS315   LEU2        U03441

I don't know if these are the same vectors you are using, but hese are
very commonly used.

You can BLAST search the yeast genome at:
http://genome-www2.stanford.edu/cgi-bin/SGD/nph-blast2sgd

Use the vector sequences to search the yeast genome, and look for the
matches at the genes you are interested in (click on "ORF map to see
where the match is).  From there you can determine the extent and sequence
of the promoter region.

Alex



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