integration of GFP tag

Marjon A.van Slegtenhorst MA_vanSlegtenhorst at fccc.edu
Wed Jul 3 15:05:23 EST 2002


I want to design primers to intergrate a GFP tag C-term of my gene from
the pFA6a-GFP(S65T)-kanMX6 plasmid, but I'm not sure how far the two
gene specific sequences should be apart to get a successful integration.
I want to delete as little as possible from the endogenous 3' region...
Thanks for the advise..
Marjon
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