Peter Kosa pkosa at
Sat Jul 20 09:58:42 EST 2002

Hi everybody,
I want to use blasticidine cassette for PCR gene disruption in yeast. I would like to known how to plate transformants. First non-selective medium (how long?) and then selective (containing blasticidin) or direct plating transformatns on selective medium with antibiotic?

Peter Kosa
Department of Biochemistry
Faculty of Natural Sciences
Comenius University
Mlynska dolina CH-1
84215 Bratislava
E-mail: pkosa at

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