Untransformed AH109 Turns Blue!

Michael Hunter mhunter0 at jomail.jo.ecu.edu.au
Sun Jul 28 21:20:25 EST 2002


Hi,
When I streakout AH109 from a glycerol stock and grow overnight on YPD
agar, I then perform a X-gal filter lift assay and find that some of
these colonies turn blue. How can this be? given that it is an
untransformed strain?...are these spontaneous mutants or a contaminating
strain? (all colonies on the plate look uniform and are the same in
appearance).
The assay itself seems to be working, as my positive control and
negative controls, do as they are supposed to do.
Has anybody else observed this?
Cheers

Michael Hunter
Centre for Human Genetics
Edith Cowan University
100 Joondalup Drive
Joondalup
WESTERN AUSTRALIA
6027
Ph. 9400 5716
Mob. 0419 367 091
Fax. 9400 5871
http://www.chg.ecu.edu.au/people/students/michael_hunter.html
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