Use of nalgene 12l Culture Bottles

Jon Swaffield jon_swaffield at
Fri Jun 7 10:17:13 EST 2002

Dear Yeasters,  does anyone have experience with growing yeast in Nalgene 12
liter culture vessels with or without mixing and aeration?  I'm looking into
using them for growing up large batches of yeast for protein purification,
but have not found any references.

Jonathan C. Swaffield,
Assistant Professor,
Dept. of Genetics,
North Carolina State University,
3513 Gardner Hall,
Raleigh, NC 27695-7614

Tel.       919-515-5733
Fax.     919 515-3355
Email:  jon_swaffield at

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