[ISO-8859-1] Re: strong GAL4 expression AND[ISO-8859-1][ISO-8859-1]

Alex Brands abbrands at artsci.wustl.edu
Sat Jun 29 14:33:20 EST 2002


On 29 Jun 2002 e.glastetter at dkfz-heidelberg.de wrote:
> Dear yeast colleagues,
>
> I would like to induce 3 different proteins with galactose in S.cerevisiae.
> Therefore I'm looking for a yeast strain or construct which harbours the GAL4
> transcription factor under the control of a strong constitutive promoter, which
> I would like to integrate into my yeast strain.

I think you may be confused about how this works.  If your intention is to
induce gene expression with galactose, you simply need to clone that gene
in front of a galactose inducible promoter, such as the GAL1 promoter.  To
induce expression, transfer the cells to media that contains galactose and
lacks glucose.  I don't see why consitutive GAL4 expression would be
required.

Alex

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