glu-ala repeat

Suzanne McKenna smckenna at cognosci.com
Tue Dec 16 14:13:44 EST 2003


I have expressed a protein in P Pastoris using the alpha factor mating
signal followed by a Glu-Ala repeat for secretion. The Glu-Ala repeat
was not cleaved from the amino terminus of the protein. Is there
anything I can do to facilitate cleavage - change in media, pH, ... to
fix this? Or is this a steric issue I can't get around. Will
fermentation vs shaker flask expression affect cleavage of the Glu-Ala
repeat?

thanks,
Suzanne 

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