Question regarding glycerol+ethanol+succinate medium

[iso-8859-1] Björn Johansson bjorn_johansson at bio.uminho.pt
Mon Jun 23 10:44:30 EST 2003


Hi,

I am routinely using a rich medium containing: 

yeast extract
peptone
glycerol
ethanol
succinate

To select S. cerevisiae against petite formation and to check
respiratory growth in general.

I wonder if someone can tell me what the ethanol and the succinate is
for?
Shouldn’t only glycerol be just fine ?

Sincerely,
Björn Johansson 


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 Björn Johansson
 Departamento de Biologia
 Universidade do Minho
 4710-057 Braga
 Portugal

 email  bjorn_johansson at bio.uminho.pt
 Phone (Mob. ) +46 705 83 30 52
 Phone (Dept.) +351-253604310
 Fax   (Dept.) +351-253678980
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