S. cerevisiae ascus staining

[Jeff Thompson]

Hi,

Does anyone have a reliable protocol for staining S. cerevisiae asci?  
I'm looking for something fairly simple with minimal hazardous reagents 
that could be used for a lab class exercise.  I've seen mention of the 
use of malachite green online, but I haven't been able to locate any 
detailed protocols.

Thanks,
Jeff Thompson

-- 
Jeff Thompson, Ph.D.
Assistant Professor of Biology
Talbot Hall 321
Denison University
Granville, OH 43023
740-587-5581

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