RE choice

Joseline Felix-MInnaar FelixMinnaarJ at pentech.ac.za
Thu Feb 5 10:05:30 EST 2004


Hi all
I need to digest the yeast genome for separation on a gel before
probing for my inserted gene of interest.  I know which enzymes I do not
want to use (anything that cuts within my gene is obviuosly not on) how
do i decide which enzyme to use. Any more scientific approach than
picking a few 6bp cutters from the endless list and see what the outcome
is? 
 
Help would be appreciated thanx.
Joseline
 
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