New TAP fusion library now available

Troy Moore tmoore at openbiosystems.com
Thu Jan 22 16:38:30 EST 2004


TO THE YEAST RESEARCH COMMUNITY:
 
I'm writing to announce the launch of the Yeast TAP-Fusion Library recently
published by Drs. E. O'shea and J. Weissman (Nature 425: 737-741).  In
keeping our committment to expanding access to community resources, this
arrayed library is available to yeast labs around the world.  
 
Technicals details on the Yeast TAP-Fusion clones (scTAP): 
 
-Individual clones are available for over 98% of the annotated yeast genes
 
-The tandem affinity purification (TAP) tag permits rapid, gentle
purification of protein complexes.
 
-Each gene is fused with high-affinity epitope tags (Calmodulin Binding
Protein & Protein A) and is expressed in it's natural chromosome location
under natural promoters.
 
-All clones have been re-confirmed via PCR with gene specific primers.
 
-The scTAP clones can be obtained on a gene-by-gene basis or in microtiter
plates
 
More information on the scTAP library is available at:
http://www.openbiosystems.com/yeast-tap-fusion-library.php
 
Troy Moore
tmoore at openbiosystems.com
 
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