[Yeast] Mating question

Noa Rappaport NRAPPAPO at wisemail.weizmann.ac.il
Tue Jul 26 04:02:09 EST 2005


Hello,
 
I would like to mate yeast on plates. It's important to me that they
stay in place once the mating begins. I thought to do this by gluing
them to the surface by polylysine on a glass bottom dish and adding
medium. I was wandering whether anyone has had an experience with such a
thing. Is it possible ? How can I increase the efficiency ? Will I have
to use YPD or will SC be fine ? Any ideas for the protocol ?
 
Thanks,
 
 

===============================
Noa Rappaport
Dept. of Molecular Genetics
Weizmann Institute of Science
noa.rappaport at weizmann.ac.il 
Tel.:  +972-8-934-4907
FAX:   +972-8-934-4108
Cell.:  054-4982014
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