[Yeast] Pulse-Field Gel Electrophoresis Equipment
david.bonfil at weizmann.ac.il
Wed Jul 27 03:34:59 EST 2005
WE are using the pulse field gel electrophoresis equipment from BioRad .
We are trying to measure the size of linear BAC (bacterial artificial
chromosome) according to Lambda marker . At first , the size of the BAC
according to the marker was smaller than we accepted .
Next , we added a control BAC as a marker and run the Pulse field again
with the lambda marker .
Again the marker did not indicated to the right size .
We tried to run all the samples in a different Pulse field equipment .
The results were completely different from the two first trials . Still
the marker run in a different format but in the right position
according to the BAC marker .
After a long story my question is :
Why the marker runs differently ,and by that i mean , in one apparatus
the right way and the other not even remotely close to the right size
of the BAC marker?
Department of Molecular Genetics
Weizmann Institute of Science
76100 Rehovot, Israel
email: david.bonfil at weizmann.ac.il
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