[Yeast] GFP photobleaching problem

Mehmet Cetin cetin.m at gmail.com
Sat Sep 3 18:39:37 EST 2005


hi all,

I am having problems with GFP expressing yeast (S.cerevisae) cells. i
observe a fixed region on the slide for an hour and a half. i excite
the cells for every 10 min (300 milliseconds excitations) to take
images. but they lose fluorescence after a few excitations. GFP seems
to photo bleach. any solutions for this problem?  (rather than
decreasing excitation time)

thanks

Mehmet Cetin



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