[Yeast] (no subject)

Rasha Nassar rasha_nassar10 at yahoo.com
Fri Jun 23 07:23:49 EST 2006

  i am doing aproject with yeasts. these yeasts contain a temp. sensitive plasmid(PP805.3) at 37.(the plasmid contain a gene that nessecary to yeast polifiration).
  i did a transformation to these yeasts with non_sensitive plasmid that containe the same gene. 
  i expected that the yeasts now will grow at 37 but they did not although i am sure that the transformation was succed .
   how can i explain this and what should i do to continue.
  thanks alot................

Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls.  Great rates starting at 1¢/min.
-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://www.bio.net/bionet/mm/yeast/attachments/20060623/ee706b60/attachment.html

More information about the Yeast mailing list