[Yeast] growth on nonfermatable carbon source

vipulkumar parmar via yeast%40net.bio.net (by vipulgreat At gmail.com)
Thu Nov 23 06:24:29 EST 2006


i have integrated a SSN6 domain with ADH1 promoter and hCMV promoter into
the genome of yeast.For my further expts i need to chek their growth on
nonfermantable carbo source.but i have doneintegrative  transformation at
least 4 times succesfully ,but then they dont grow on growth on
nonfermatable carbon source.

So my query is that ,Does the integration of SSN6 into the yeast genome
affect such phenotype that it renders no growth  on nonfermatable carbon
source???(because SSN6 is a genral transcrition co repressor).

Thanks

You can reply me at vipulgreat At gmail.com
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