[Yeast] DAPI staining

lijo john via yeast%40net.bio.net (by indianyeast from gmail.com)
Thu Oct 12 08:15:00 EST 2006

hi all

              Im trying to use the DAPI staining for budding yeast
saccharomyces cerevisae  BY4742 , but the dye is not entering the yeast
cells .
              Is it better  to prepare spheroplast  befroe incubation with
DAPI ? or DAPI is permeable to live yeast cells .

                please help me
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