[Yeast] After yeast two-hybrid?
(by aurelien.boland from fundp.ac.be)
Tue Aug 7 08:51:06 EST 2007
I've realized a yeast two-hybrid screen and found some potential preys.
I've some problems in doing GST pull-down Assay
and Co-immunoprecipitation is not possible.
I'm able to produce bait and preys in vitro and
to detect them after western blot
I would like to know if my idea is good or not...
My idea is to realize something like an EMSA: A
polyacryamide gel and to see if there's a shift
of a band after incubing prey and bait.
The detection will be done by western blot.
Thanks for your responses,
e-mail: aurelien.boland from fundp.ac.be
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