[Yeast] diauxic shift - when?

Nicolas Brandes via yeast%40net.bio.net (by nbrandes from umich.edu)
Mon Aug 13 10:48:59 EST 2007


Hi to all,
Can one say when the diauxic shift in SDC medium occurs? (OD-wise?). I'm
using a EG103 strain (MATα leu2-3, 112 hisΔ1 trp1-289 ura3-52 GAL+), and I'm
wondering if the diauxic shift already occurs at low ODs or at the point
where the glucose is depleted (I don't want to measure this) and/or the
culture slows down its exponential phase growth? My SDC medium consist of
YNB, all the AA and 2% glucose. 
 
I appreciate any help,
Nicolas Brandes
 
 
-----
Nicolas Brandes
University of Michigan
Department of MCDB
 
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