[Yeast] Glycerol stocks from cultures in 2xYPAD

Anna Heidenblut via yeast%40net.bio.net (by anna.heidenblut At gmx.de)
Fri Jan 19 05:43:50 EST 2007


Dear all,

another elementary question:
Can it cause any problems when I prepare glycerol stocks from cultures 
that were grown in double strength YPAD (YPD with additional adenin 
added)? I use this 2xYPAD to grow pre-cultures for yeast transformation 
and it would be very convenient to prepare glycerol stocks from the 
pre-cultures immediately rather than setting up a separate culture in 
YPD just for the preparation of glycerol stocks.

Any comments on that question are highly appreciated.

Anna

-- 
Dr. Anna Heidenblut (PhD)

Genome Integrity Group
Department of Molecular Oncology
Weatherall Institute of Molecular Medicine
Oxford University John Radcliffe Hospital,
Headley Way, Oxford, Oxfordshire OX3 9DS UK
Tel: 0044 / 1865 / 222419
anna.heidenblut At cancer.org.uk



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