[Yeast] 3-at

luca.lotito3 via yeast%40net.bio.net (by luca.lotito3 from unibo.it)
Mon May 14 04:01:20 EST 2007


 

Hi,

i want to use 3-at to analyzed silencing of his5 gene of s.pombe cloned at
telomere of chromosome XIV of. S. cerevisiae.

Someone have a protocols to use that drug? I have test it but the yeast did
not die at 200mM of 3-at. I test the drug also in other strains with wt his3
gene, but the results are the same: the yeast did not die.

Someone help me?

thanks

++++++++++++++++++++++++++++++++++++++++++

Luca Lotito, PhD

Department of Biochemistry, University of Bologna

via Irnerio 48, 40126 Bologna, Italy

Phone: +39 051 2094282 

Fax: +39 051 2094283

 <http://www.biotecnologie.unibo.it/capranicolab/index.php>
http://www.biotecnologie.unibo.it/capranicolab/index.php

 

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