[Yeast] 3-at

[John Bida]

Are you using -HIS drop out media with 3-AT?

~Jp Bida




On May 14, 2007, at 4:01 AM, luca.lotito3 wrote:

>
> Hi,
>
> i want to use 3-at to analyzed silencing of his5 gene of s.pombe  
> cloned at telomere of chromosome XIV of. S. cerevisiae.
>
> Someone have a protocols to use that drug? I have test it but the  
> yeast did not die at 200mM of 3-at. I test the drug also in other  
> strains with wt his3 gene, but the results are the same: the yeast  
> did not die.
>
> Someone help me?
>
> thanks
>
> ++++++++++++++++++++++++++++++++++++++++++
>
> Luca Lotito, PhD
>
> Department of Biochemistry, University of Bologna
>
> via Irnerio 48, 40126 Bologna, Italy
>
> Phone: +39 051 2094282
>
> Fax: +39 051 2094283
>
> http://www.biotecnologie.unibo.it/capranicolab/index.php
>
>
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