(by Bida.John from mayo.edu)
Tue May 15 10:01:48 EST 2007
Are you using -HIS drop out media with 3-AT?
On May 14, 2007, at 4:01 AM, luca.lotito3 wrote:
> i want to use 3-at to analyzed silencing of his5 gene of s.pombe
> cloned at telomere of chromosome XIV of. S. cerevisiae.
> Someone have a protocols to use that drug? I have test it but the
> yeast did not die at 200mM of 3-at. I test the drug also in other
> strains with wt his3 gene, but the results are the same: the yeast
> did not die.
> Someone help me?
> Luca Lotito, PhD
> Department of Biochemistry, University of Bologna
> via Irnerio 48, 40126 Bologna, Italy
> Phone: +39 051 2094282
> Fax: +39 051 2094283
> Yeast mailing list
> Yeast from net.bio.net
-------------- next part --------------
An HTML attachment was scrubbed...
More information about the Yeast