[Yeast] 3-at

John Bida via yeast%40net.bio.net (by Bida.John from mayo.edu)
Tue May 15 10:01:48 EST 2007


Are you using -HIS drop out media with 3-AT?

~Jp Bida




On May 14, 2007, at 4:01 AM, luca.lotito3 wrote:

>
> Hi,
>
> i want to use 3-at to analyzed silencing of his5 gene of s.pombe  
> cloned at telomere of chromosome XIV of. S. cerevisiae.
>
> Someone have a protocols to use that drug? I have test it but the  
> yeast did not die at 200mM of 3-at. I test the drug also in other  
> strains with wt his3 gene, but the results are the same: the yeast  
> did not die.
>
> Someone help me?
>
> thanks
>
> ++++++++++++++++++++++++++++++++++++++++++
>
> Luca Lotito, PhD
>
> Department of Biochemistry, University of Bologna
>
> via Irnerio 48, 40126 Bologna, Italy
>
> Phone: +39 051 2094282
>
> Fax: +39 051 2094283
>
> http://www.biotecnologie.unibo.it/capranicolab/index.php
>
>
> _______________________________________________
> Yeast mailing list
> Yeast from net.bio.net
> http://www.bio.net/biomail/listinfo/yeast

-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://www.bio.net/bionet/mm/yeast/attachments/20070515/35a3725c/attachment.html


More information about the Yeast mailing list