[Yeast] Yeast spheroplast RT-PCR
(by WangXX from MedImmune.com)
Thu May 31 19:29:43 EST 2007
Has anyone done RT-PCR from spheroplast? I mean using spheroplast as template without extracting total RNA. Originally I just used water to lyze spheroplast but all of a sudden my RTPCR has stopped working. This is telling me I have been working on the limit. Any idea how to eliminate the genomic DNA/cellular protein contaminant while keeping the integrity of mRNA? Thanks!
BTW, RT=reverse transcription.
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