From jejasmin from ucsd.edu Wed Nov 14 05:38:11 2007 From: jejasmin from ucsd.edu (Jean-Nicolas Jasmin) Date: Wed Nov 14 10:55:43 2007 Subject: [Yeast] pombe respiration Message-ID: <200711132235.lADMZu5c003112@biomail.ucsd.edu> Hi, I am working with Sz pombe strains FY261 and FY254 and I find it difficult to grow them on a non-fermentable substrate. I have tried solid media with yeast extract (1 to 5%) and glycerol (1 to 3%) and ethanol (1 to 3%), plus amino acids. On 5% YE and 2% glycerol and ethanol it takes about 2 weeks to get large colonies and the colonies show very high variation in size. Is this normal for this genotype? Which pombe strain should I use to obtain better growth on non-fermentable substrates? Many thanks, Jean-Nicolas EBE, Biological Sciences, UCSD From I.Jourdain from massey.ac.nz Wed Nov 14 15:06:50 2007 From: I.Jourdain from massey.ac.nz (Isabelle Jourdain) Date: Wed Nov 14 18:15:25 2007 Subject: [Yeast] RE: Yeast Digest, Vol 30, Issue 1 In-Reply-To: <200711141705.lAEH57Y18712@net.bio.net> Message-ID: <200711142006.JAA29705@its-mail1.massey.ac.nz> Hello Jean Nicolas, In fission yeast, there is no variation in the genetic background, like in budding yeast, and I'd say changing the strains is not going to make much difference. I don't know what FY261 and FY254 are (probably the stock numbers of some guy whose initials are FY). Just use any WT as a control, as long as the cells look happy, you're good. Only, if you want to compare various mutants, try to use strains that have the same auxotrophy markers. I was told, and it works, to prepare the glucose-free plates by adding 0.1% glucose anyway. The cells need it to initiate there growth, they use it all, and then start using the other source of carbon. It nevertheless takes ages to see the first colonies. I have never noticed any variation in colony size and cannot help you on this matter. I hope this helps. Very good luck, Isabelle -----Original Message----- From: yeast-bounces@oat.bio.indiana.edu [mailto:yeast-bounces@oat.bio.indiana.edu] On Behalf Of yeast-request@oat.bio.indiana.edu Sent: Thursday, 15 November 2007 6:05 a.m. To: yeast@magpie.bio.indiana.edu Subject: Yeast Digest, Vol 30, Issue 1 Send Yeast mailing list submissions to yeast@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/yeast or, via email, send a message with subject or body 'help' to yeast-request@net.bio.net You can reach the person managing the list at yeast-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Yeast digest..." Today's Topics: 1. pombe respiration (Jean-Nicolas Jasmin) ---------------------------------------------------------------------- Message: 1 Date: Wed, 14 Nov 2007 02:38:11 -0800 From: Jean-Nicolas Jasmin Subject: [Yeast] pombe respiration To: yeast@magpie.bio.indiana.edu Message-ID: <200711132235.lADMZu5c003112@biomail.ucsd.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Hi, I am working with Sz pombe strains FY261 and FY254 and I find it difficult to grow them on a non-fermentable substrate. I have tried solid media with yeast extract (1 to 5%) and glycerol (1 to 3%) and ethanol (1 to 3%), plus amino acids. On 5% YE and 2% glycerol and ethanol it takes about 2 weeks to get large colonies and the colonies show very high variation in size. Is this normal for this genotype? Which pombe strain should I use to obtain better growth on non-fermentable substrates? Many thanks, Jean-Nicolas EBE, Biological Sciences, UCSD ------------------------------ _______________________________________________ Yeast mailing list Yeast@net.bio.net http://www.bio.net/biomail/listinfo/yeast End of Yeast Digest, Vol 30, Issue 1 ************************************ From mks131 from gmail.com Thu Nov 15 06:38:25 2007 From: mks131 from gmail.com (Manoj saxena) Date: Thu Nov 15 10:39:06 2007 Subject: [Yeast] Yeast nuclease Message-ID: <78e7890b0711150338q1c817edftbcc0c1f0298acac1@mail.gmail.com> Do anybody have a complete list of yeast nucleases. I have an old stock of yeast nuclear extract but it have nuclease contamination. Can anyone please suggest me how to remove this contamination. THANKs From kechiafred from yahoo.com Mon Nov 19 05:32:37 2007 From: kechiafred from yahoo.com (Frederick Kechia) Date: Mon Nov 19 10:31:11 2007 Subject: [Yeast] inquiry Message-ID: <867342.41023.qm@web90401.mail.mud.yahoo.com> Dear Sir/Madam, I am cameroonian writing from Cameroon. I am an assistant lecturer of Medical Mycology in the faculty of Medicine and biomedical sciences University of Yaounde I, Cameroon. I am very interested in studying the molecular biology of yeast. I have some experience in molecular technics done on cryptococcus species which I got in the Molecular Biology laboratory of the Mycology unit of the National institute for communicable diseases, Johanesburg, South africa. I am looking for an opportunity to acquire more experience on ther yeast. I would be very grateful if you could furnish me with information on how I can join your laboratory. Best regards and thank you in anticipation. FA Kechia,(PhD) Frederick A. KECHIA (Ph.D.) Mycology Laboratory Faculty of Medicine and Biomedical Sciences UNIVERSITY of YAOUNDE I CAMEROON Tel 237 7776 29 37 ____________________________________________________________________________________ Be a better pen pal. Text or chat with friends inside Yahoo! Mail. See how. http://overview.mail.yahoo.com/ From s22076744 from tuks.co.za Tue Nov 20 03:55:51 2007 From: s22076744 from tuks.co.za (Pieter Swanepoel) Date: Tue Nov 20 11:36:18 2007 Subject: [Yeast] Sucrose fermentation Message-ID: <20071120105551.9h9coeyzs48cs4c8@student.up.ac.za> Hi I am looking for a recipe to ferment sucrose as the main carbon source for Saccharomyces cerevisiae. Does anybody have one? I'd like it to be Urea-free. Regards Pieter Swanepoel This message and attachments are subject to a disclaimer. Please refer to www.it.up.ac.za/documentation/governance/disclaimer/ for full details. / Hierdie boodskap en aanhangsels is aan 'n vrywaringsklousule onderhewig. Volledige besonderhede is by www.it.up.ac.za/documentation/governance/disclaimer/ beskikbaar. From gabe.musso from utoronto.ca Tue Nov 20 15:06:26 2007 From: gabe.musso from utoronto.ca (Gabriel Musso) Date: Tue Nov 20 15:40:06 2007 Subject: [Yeast] waltii question Message-ID: <47433E42.80403@utoronto.ca> Hello, For several weeks I've been trying various types of media to induce sporulation in Kluyveromyces waltii (aka Lachancea waltii) but nothing seems to work. Does anyone have any experience with this species? Thanks. Gabe -- Gabriel Musso, PhD Candidate Genome Biology & Bioinformatics Collaborative Program Department of Molecular Genetics University of Toronto From khartwi from gwdg.de Wed Nov 21 11:41:46 2007 From: khartwi from gwdg.de (Katrin Hartwich) Date: Wed Nov 21 13:15:19 2007 Subject: [Yeast] Mating type switching in S. cerevisiae Message-ID: <33999.134.76.207.1.1195663306.squirrel@mailbox.gwdg.de> Hello to all, I'm working with the S. cerevisiae strains from the EUROSCARF collection (BY4741). All strains have the mating type a and I want to switch now the mating type of some of them to alpha. I have a protocol and a plasmid for mating type switching from a friend but it didn't work that well. Does anybody have a good working protocol/plasmid for mating type switching for me? Thank's a lot! Best wishes, Katrin -- Katrin Hartwich European Neuroscience Institute G?ttingen (ENI-G) Molecular Neurogenetics Grisebachstra?e 5 37073 G?ttingen Germany Phone: +49 (0)551-39-13972 Fax: +49 (0)551-39-12346 Email: khartwi@gwdg.de From Els from w3z.co.uk Wed Nov 28 14:20:20 2007 From: Els from w3z.co.uk (Eleanor Compton) Date: Fri Nov 30 10:53:17 2007 Subject: [Yeast] Yeast effected by dog food? Message-ID: <000a01c831f3$b7d3bca0$de54fea9@Marvin> Hi. I wouldnt normally send emails to people i dont know out of the blue so im sorry, but i need help. I'm currently doing my biology AS coursework on the effects of certain substances on yeast respiration, using the breaking down of glucose for the reaction and dcpip indicater to see the rate of reaction. I've used Iams dog biscuits with the ingredients Chicken, chicken by-product meal, corn meal, ground whole grain sorghum, ground whole grain barley, chicken meal, chicken fat (preserved with mixed tocopherols, a source of vitamin E, and citric acid), fish meal (source of fish oil), dried beet pulp (sugar removed), natural chicken flavor, dried egg product, brewers dried yeast, potassium chloride, salt, flax meal, choline chloride, ferrous sulfate, dicalcium phosphate, dl-methionine, zinc oxide, vitamin E supplement, ascorbic acid, manganese sulfate, copper sulfate, manganous oxide, vitamin A acetate, calcium pantothenate, biotin, rosemary extract, thiamine mononitrate (source of vitamin B1), vitamin B12 supplement, niacin, riboflavin supplement (source of vitamin B2), inositol, pyridoxine hydrochloride (source of vitamin B6), vitamin D3 supplement, potassium iodide, folic acid, cobalt carbonate When i did the experiment it slowed down the reaction of the dcpip indicater, but this doesnt nessercarly mean it was to do with yeast respiration. Does anyone know if any of these ingredience effect the respiration of yeast or enzymes used in any way? I'd be so greatful if anyone had any useful information, >From eleanor. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20071128/32df903d/attachment.html