[Yeast] RE: Yeast Digest, Vol 30, Issue 1

Isabelle Jourdain via yeast%40net.bio.net (by I.Jourdain from massey.ac.nz)
Wed Nov 14 15:06:50 EST 2007


Hello Jean Nicolas,

In fission yeast, there is no variation in the genetic background, like in
budding yeast, and I'd say changing the strains is not going to make much
difference. I don't know what FY261 and FY254 are (probably the stock
numbers of some guy whose initials are FY). Just use any WT as a control, as
long as the cells look happy, you're good. Only, if you want to compare
various mutants, try to use strains that have the same auxotrophy markers.

I was told, and it works, to prepare the glucose-free plates by adding 0.1%
glucose anyway. The cells need it to initiate there growth, they use it all,
and then start using the other source of carbon. It nevertheless takes ages
to see the first colonies. I have never noticed any variation in colony size
and cannot help you on this matter. 

I hope this helps.

Very good luck,

Isabelle




-----Original Message-----
From: yeast-bounces from oat.bio.indiana.edu
[mailto:yeast-bounces from oat.bio.indiana.edu] On Behalf Of
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Sent: Thursday, 15 November 2007 6:05 a.m.
To: yeast from magpie.bio.indiana.edu
Subject: Yeast Digest, Vol 30, Issue 1

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Today's Topics:

   1. pombe respiration (Jean-Nicolas Jasmin)


----------------------------------------------------------------------

Message: 1
Date: Wed, 14 Nov 2007 02:38:11 -0800
From: Jean-Nicolas Jasmin <jejasmin from ucsd.edu>
Subject: [Yeast] pombe respiration
To: yeast from magpie.bio.indiana.edu
Message-ID: <200711132235.lADMZu5c003112 from biomail.ucsd.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

Hi,

I am working with Sz pombe strains FY261 and FY254 and I find it 
difficult to grow them on a non-fermentable substrate. I have tried 
solid media with yeast extract (1 to 5%) and glycerol (1 to 3%) and 
ethanol (1 to 3%), plus amino acids. On 5% YE and 2% glycerol and 
ethanol it takes about 2 weeks to get large colonies and the colonies 
show very high variation in size.

Is this normal for this genotype?

Which pombe strain should I use to obtain better growth on 
non-fermentable substrates?

Many thanks,

Jean-Nicolas
EBE, Biological Sciences, UCSD



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