[Yeast] declumping yeast cells for FACS analysis

[Aneesh Panoli]

Dear colleagues,
I need to analyze cells based on DNA content at various times after starting sporulation. I'm using an SK1 background. I find a lot of clumping even after fixation and sonication of cells. I find that cells separate on sonication but come together again. Is there a treatment that will separate the cells and keep them apart?
Thanks,
Aneesh Panoli
------------------------------------------------------------
Aneesh P. Panoli
E-307
Centre for Cellular and Molecular Biology
Uppal Road, Hyderabad-500007
India
Tel: +91-40-27192647
Fax:+91-40-27160591
E-mail: aneeshpanoli from gmail.com 
------------------------------------------------------------
-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://www.bio.net/bionet/mm/yeast/attachments/20071023/cdaa8b28/attachment.html