From manu3989 from gmail.com Tue Jan 1 23:43:42 2008 From: manu3989 from gmail.com (Mohan T.C) Date: Sat Jan 5 16:34:05 2008 Subject: [Yeast] Please Help me Message-ID: Dear Sir, I am trying to express plant codon optimized Cry1Ac gene in yeast strain INVSc1,under the control of galactose inducible promoter.but i am expected band in SDS PAGE.please can you suggest me how to prepare yeast cells to do SDS analysis.i read in some articles that cry1Ac forms inclusion bodies,so i am not getting how to analyse this thing, please give me a detailed procedure i am waiting for your response Thanking You Mobile:09900484917 MOHAN TC M.Sc(Agri)Biotechnology, Institute of Agri Biotechnology(IABT) University of Agricultural Sciences. Dharwad - 580005. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20080102/1bde51ef/attachment.html From jerica.sabotic from ijs.si Fri Jan 11 09:46:43 2008 From: jerica.sabotic from ijs.si (Jerica Sabotic) Date: Fri Jan 11 10:22:03 2008 Subject: [Yeast] pEG(KG) vector sequence Message-ID: <7.0.0.16.1.20080111154522.01841b48@ijs.si> Hello, Could any one please send me the pEG(KG) vector sequence ? Thank you in advance, Jeri Dr. Jerica Sabotic Department of Biotechnology Jozef Stefan Institute Jamova 39 SI-1000 Ljubljana Slovenia Tel: +386 1 477 3373 Fax: +386 1 477 3984 From tim.dybvig from adimab.com Tue Jan 15 17:46:19 2008 From: tim.dybvig from adimab.com (Tim Dybvig) Date: Tue Jan 15 23:57:47 2008 Subject: [Yeast] Job Opportunities - Adimab, Inc. Message-ID: <478d37c9.1aab7e0a.7b5b.108e@mx.google.com> Become part of an energetic group of scientist committed to building a cutting edge biotechnology company. RESPONSIBILITIES Support the development of a novel antibody discovery, maturation and production platform including: - implementing novel yeast surface presentation systems - realizing discovery strategies for human antibodies in yeast - enhancing the performance of antibody expression in yeast REQUIREMENTS - Ph.D. / M.S. / Bachelors degree in the Life Sciences - Expertise in Molecular or Cell Biology, Yeast Genetics, Protein Engineering, or Fermentation Science - Must be motivated, passionate and committed individual who works well on a team - Previous yeast, phage or other display platform experience preferred - Other desirable skills include FACs analysis/sorting, protein expression, purification and characterization, and Yeast Genetics. ABOUT ADIMAB Adimab is changing the discovery, maturation and production of fully human therapeutic antibodies. Adimab was founded by two of the world's leading yeast biotechnologists: Professor Tillman Gerngross (Dartmouth, Co-founder of GlycoFi, Inc., a wholly owned subsidiary of Merck & Co. since 2006), and Professor Dane Wittrup (MIT, Co-founder of Biodisplay, acquired by Abbott Labs in 2001). Adimab's hiring process focuses on the quality of individual contributors and their ability to integrate into a highly energetic and fast paced team of scientists. Adimab will only make offers to the very best candidates following a rigorous interview and review process - compensation is above industry average and includes meaningful equity participation. For more go to www.adimab.com/careers, or contact hr@adimab.com. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20080115/f352ede2/attachment.html From mks131 from gmail.com Mon Jan 21 04:27:59 2008 From: mks131 from gmail.com (Manoj saxena) Date: Mon Jan 21 11:23:51 2008 Subject: [Yeast] gene interaction maps Message-ID: <78e7890b0801210127m50e35a3bt4cf81111fcd025e2@mail.gmail.com> Hi all I am new to bioinformatics. If anyone can help me to locate the databases and programs to predict the yeast gene/ protein interaction maps. Thanks Manoj W313 CCMB Hyderabad-500007 India -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20080121/a4017e1f/attachment.html From linhe from sbc.su.se Thu Jan 24 16:17:25 2008 From: linhe from sbc.su.se (Linnea Hedin) Date: Thu Jan 24 16:57:34 2008 Subject: [Yeast] Unwanted digestion of solubilized membrane proteins. Message-ID: <47990065.8060607@sbc.su.se> An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20080124/fe667810/attachment.html From Martin.Kampmann from mail.rockefeller.edu Fri Jan 25 12:28:42 2008 From: Martin.Kampmann from mail.rockefeller.edu (Martin Kampmann) Date: Fri Jan 25 12:46:05 2008 Subject: [Yeast] Re: Unwanted digestion of solubilized membrane proteins. In-Reply-To: <200801251707.m0PH7XL03925@net.bio.net> References: <200801251707.m0PH7XL03925@net.bio.net> Message-ID: <4CBFABEC-9562-40F2-80A7-3F8C3145C39A@rockefeller.edu> Hi Linnea, Degradation may occur during your 1 hour solubilization step. Maybe you could try to do 10 minutes of solubilization before the clearing step, and then analyze your sample directly after clearing? Best, Martin On Jan 25, 2008, at 12:07 PM, yeast-request@oat.bio.indiana.edu wrote: > Send Yeast mailing list submissions to > yeast@net.bio.net > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/yeast > or, via email, send a message with subject or body 'help' to > yeast-request@net.bio.net > > You can reach the person managing the list at > yeast-owner@net.bio.net > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Yeast digest..." > > > Today's Topics: > > 1. Unwanted digestion of solubilized membrane proteins. > (Linnea Hedin) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 24 Jan 2008 22:17:25 +0100 > From: Linnea Hedin > Subject: [Yeast] Unwanted digestion of solubilized membrane proteins. > To: yeast@magpie.bio.indiana.edu > Message-ID: <47990065.8060607@sbc.su.se> > Content-Type: text/plain; charset="us-ascii" > > An HTML attachment was scrubbed... > URL: http://www.bio.net/bionet/mm/yeast/attachments/20080124/ > fe667810/attachment-0001.html > > ------------------------------ > > _______________________________________________ > Yeast mailing list > Yeast@net.bio.net > http://www.bio.net/biomail/listinfo/yeast > > End of Yeast Digest, Vol 32, Issue 5 > ************************************ From Valiveti from Biology.Rutgers.Edu Mon Jan 28 14:15:58 2008 From: Valiveti from Biology.Rutgers.Edu (Valiveti, Aswani) Date: Mon Jan 28 14:40:24 2008 Subject: [Yeast] His3 background test Message-ID: <03677301F5079A40BBC85B346EE23DD038A7063B03@exch07g4.ad.lifesciences.rutgers.edu> Hi, I am performing a one hybrid experiment using pHisi-1. pLaczi reporter vectors and pJG4-5-cDNA library in EGY48 strain. While testing for background His3 reporter expression I find that the yeast grow even at 125mM 3-AT. The strains with plasmid alone also grow at 60mM 3-AT, further concentrations not tested yet. How high concentration of 3-AT can I use to completely eradicate the growth due to leaky expression. What are the effects of 3-AT on yeast growth, aside from Histidine biosynthetic pathway? Will changing the yeast host strain help in reducing the background? Will greatly appreciate any helpful advice. Thanks for your attention. regards, Aswani. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20080128/51e5e487/attachment.html