[Yeast] Please Help me

Mohan T.C via yeast%40net.bio.net (by manu3989 from gmail.com)
Tue Jan 1 23:43:42 EST 2008


Dear Sir,
I am trying to express plant codon optimized Cry1Ac gene in yeast strain
INVSc1,under the control of  galactose inducible promoter.but i am expected
band in SDS PAGE.please can you suggest me how to prepare yeast cells to do
SDS analysis.i read in some articles that cry1Ac forms inclusion bodies,so i
am not getting how to analyse this thing, please give me a detailed
procedure
i am waiting for your response
Thanking You

Mobile:09900484917
MOHAN TC
M.Sc(Agri)Biotechnology,
Institute of Agri Biotechnology(IABT)
University of Agricultural Sciences.
Dharwad - 580005.
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