[Yeast] Re: Unwanted digestion of solubilized membrane proteins.

Martin Kampmann via yeast%40net.bio.net (by Martin.Kampmann from mail.rockefeller.edu)
Fri Jan 25 12:28:42 EST 2008


Hi Linnea,

Degradation may occur during your 1 hour solubilization step.
Maybe you could try to do 10 minutes of solubilization before the  
clearing step, and then analyze your sample directly after clearing?

Best,
Martin


On Jan 25, 2008, at 12:07 PM, yeast-request from oat.bio.indiana.edu wrote:

> Send Yeast mailing list submissions to
> 	yeast from net.bio.net
>
> To subscribe or unsubscribe via the World Wide Web, visit
> 	http://www.bio.net/biomail/listinfo/yeast
> or, via email, send a message with subject or body 'help' to
> 	yeast-request from net.bio.net
>
> You can reach the person managing the list at
> 	yeast-owner from net.bio.net
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Yeast digest..."
>
>
> Today's Topics:
>
>    1. Unwanted digestion of solubilized membrane proteins.
>       (Linnea Hedin)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 24 Jan 2008 22:17:25 +0100
> From: Linnea Hedin <linhe from sbc.su.se>
> Subject: [Yeast] Unwanted digestion of solubilized membrane proteins.
> To: yeast from magpie.bio.indiana.edu
> Message-ID: <47990065.8060607 from sbc.su.se>
> Content-Type: text/plain; charset="us-ascii"
>
> An HTML attachment was scrubbed...
> URL: http://www.bio.net/bionet/mm/yeast/attachments/20080124/ 
> fe667810/attachment-0001.html
>
> ------------------------------
>
> _______________________________________________
> Yeast mailing list
> Yeast from net.bio.net
> http://www.bio.net/biomail/listinfo/yeast
>
> End of Yeast Digest, Vol 32, Issue 5
> ************************************



More information about the Yeast mailing list