[Yeast] tagging for overexpression

Cedric via yeast%40net.bio.net (by cedric.desmet from gmail.com)
Tue Jun 10 09:48:59 EST 2008


Hello everyone,

I would like to tag a gene C-terminally with a His6HA3 tag. How do I decide
where to insert this tag (directly before the stop codon?) and how do I
design the primers for this? Is there a website or software available?

Cheers,
Cedric De Smet
Utrecht University
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