From jinyohan from gmail.com  Tue Apr 14 13:39:52 2009
From: jinyohan from gmail.com (Yohan Jin)
Date: Wed Apr 15 08:33:28 2009
Subject: [Yeast] monitor cell lysis during cell culture
In-Reply-To: <51a674010904141128h656f7e4epf75f96f1d9014685@mail.gmail.com>
References: <51a674010904141128h656f7e4epf75f96f1d9014685@mail.gmail.com>
Message-ID: <51a674010904141139t114b3d85lf5b1c67ba2294f44@mail.gmail.com>

 Hi,

I am purifying a protein that is being secreted to culture medium, however,
I encounter significant amount of cytosolic proteins in the supe.  I guess
these are resulted from cell lysis.  If it is true, I'd like to monitor how
much yeast cell lysis is going on during exponential cell growth.

Please give me some advise/comments for markers, methods and references.  My
email is jinyohan@gmail.com

Thank you so much, in advance.


Yohan Jin
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From mukeshpyadava from gmail.com  Wed Apr 22 02:10:44 2009
From: mukeshpyadava from gmail.com (Mukesh Yadav)
Date: Fri Apr 24 08:57:14 2009
Subject: [Yeast] query for highly transformable vector
Message-ID: <36419f5a0904220010r44e01813p6b2b04e974b9567a@mail.gmail.com>

My work inclueds routine transformation experiments in yeast and E.coli.
I wonder if anybody knows about a highly transformable S.Pombe vector as
that VL6-48N of S. cerevisiae. (ref. Natalay Kouprina, vladimir N. Noskov
and Vladimir Larionov :methods in Molecular Biology, Vol 349)
-- 

-- 
Mukesh Pratap Yadava
C/O Prof. D.D. Dubey
Molecular Biology Laboratory
Department of Biotechnolgy
Faculty Building
VBS Purvanchal University
Jaunpur-222001 (Uttar pradesh)
Mob. No- +91-9451157085
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