[Yeast] No background with yeast two-hybrid screen
(by philana.fernandes08 from imperial.ac.uk)
Sat Aug 29 10:02:19 EST 2009
I have just recently performed a Y2HG screen and found that the plating volume of 200microlitres resulted in plates that were too dense - so much so, it looked like a lawn and individual colonies could not been seen at all.
I simply patched areas from this plate onto fresh plates and voila - single colonies.
Hope this helps....
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