From yewang from interchange.ubc.ca Sun Mar 8 04:07:39 2009 From: yewang from interchange.ubc.ca (Ye Wang) Date: Sun Mar 8 21:31:08 2009 Subject: [Yeast] slower growth rate of transformants Message-ID: <87CBDD55032044F2987E8CF52F4F0094@YePC> Hi, there, Does anybody know the casue of a much more slower growth rate of transformans? we introduced a 5kb heterologous ABC transporter gene in to Saccharomyces cerevisiae single mutant for a complementation experiment( integration vector, URA3 selection), the transformants did show up on selective medium but stay as a very little tiny colonies even after incubation for up to one week. any trouble shooting tips on this issue? Thanks in advance. Ye -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20090308/3371fe1f/attachment.html From Joshua.Rest from stonybrook.edu Mon Mar 16 14:07:12 2009 From: Joshua.Rest from stonybrook.edu (Joshua Rest) Date: Mon Mar 16 17:28:17 2009 Subject: [Yeast] Lab tech position available Message-ID: <87d3099f0903161207r1cfe613q52f8c2ead79cf029@mail.gmail.com> A Research Support Specialist position is available in the laboratory of Dr. Joshua Rest in the Department of Ecology and Evolution at Stony Brook University. * Brief Description of Duties:* Perform large-scale serial growth competitions in yeast. - Perform large scale serial growth competitions, including regular serial dilutions, relative fluorescence measurements, and associated data collection. - Construct and validate yeast strains; design primers; transform strains. - Perform ancillary competition validation: flow cytometry; large-scale RNA and DNA isolation; pyrosequencing. - Data analysis and integration. - General lab maintenance. *Salary:* $34,975 - $45,200 *Required Qualifications:* Bachelor's degree. Ability to demonstrate professional competence in research activities. Course work and laboratory experience with molecular biology protocols and equipment.* Preferred Qualifications:* Two years of laboratory experience either in yeast genetics or in high throughput biological analysis, such as cell culture or expression microarrays. *For additional information on the Department or its activities, please visit **http://life.bio.sunysb.edu/ee/restlab/index.htm.* For more information about this position, or to apply online, please go to: http://tinyurl.com/labtech or http://naples.cc.sunysb.edu/Admin/CampusJob.nsf/ebb5a78b9e8848868525659c0072eafe/1b090b4ec1c469f1852575710062a1f4?OpenDocument -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20090316/b1e40fea/attachment.html From cinthiait from yahoo.com Thu Mar 19 09:51:50 2009 From: cinthiait from yahoo.com (Cinzia Pagliuca) Date: Thu Mar 19 10:43:28 2009 Subject: [Yeast] Looking for a shuttle vector with GST tag. In-Reply-To: <200903171707.n2HH7Xh01323@net.bio.net> Message-ID: <465358.88948.qm@web23802.mail.ird.yahoo.com> Dears all, I' m looking for shuttle vector to perform a homologuos recombination in yeast S. cerevisiae genome. The plasmid must be tagged with GST and allow the C-terminal protein tagging (Protein-GST). Does anybody have it? looking for having positive feedback, a nice day Cinzia Pagliuca Cinzia, PhD. c/o Campus IFOM-IEO via Adamello, 16 20139 Milano, Italy.