[Yeast] Re: Yeast Digest, Vol 54, Issue 3

Alaattin Kaya via yeast%40net.bio.net (by akaya from genomics.unl.edu)
Thu Nov 26 13:55:18 EST 2009


Hi ;
Use sporulation media for your mutant strain. this is the easy way I think. 
on sporulation media if there is a spores that means your strain is somehow 
diploid. You can check under microscope.

Alaattin KAYA


----- Original Message ----- 
From: <yeast-request from oat.bio.indiana.edu>
To: <yeast from magpie.bio.indiana.edu>
Sent: Thursday, November 26, 2009 11:06 AM
Subject: Yeast Digest, Vol 54, Issue 3


> Send Yeast mailing list submissions to
> yeast from net.bio.net
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://www.bio.net/biomail/listinfo/yeast
> or, via email, send a message with subject or body 'help' to
> yeast-request from net.bio.net
>
> You can reach the person managing the list at
> yeast-owner from net.bio.net
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Yeast digest..."
>
>
> Today's Topics:
>
>   1. Phloxine B staining: diploidy or mutant phenotype?
>      (Febe van Maldegem)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 25 Nov 2009 18:53:00 +0000
> From: Febe van Maldegem <maldegem from mrc-lmb.cam.ac.uk>
> Subject: [Yeast] Phloxine B staining: diploidy or mutant phenotype?
> To: yeast from magpie.bio.indiana.edu
> Message-ID: <4B0D7D0C.8050603 from mrc-lmb.cam.ac.uk>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Dear colleagues,
>
> I have a temperature sensitive mutant strain, that also grows in red
> colonies on Phloxine B plates at 30C.
> How do I distinguish diploidy from a reduced viability due to the
> mutation? In other words, how can I reassure myself that I have a
> haploid mutant strain?
>
> Thanks in advance!
> Febe van Maldegem
> MRC-LMB, Cambridge, UK
>
>
>
> ------------------------------
>
> _______________________________________________
> Yeast mailing list
> Yeast from net.bio.net
> http://www.bio.net/biomail/listinfo/yeast
>
> End of Yeast Digest, Vol 54, Issue 3
> ************************************
> 



More information about the Yeast mailing list