[Yeast] Reply for 5-FOA

[dulu mahanty]

Hi!
When you are plating your URA+ yeast cells on  5-FOA, the chance of getting false positives which are URA+ is very low. But you will get large number of aneuploid colonies (with unwanted mutations) which are URA-. So, it is recommended that whenever you are using this compound you should check your colonies with Southern hybridization, rather than PCR.
 
regards 


sarmistha mahanty
C/O Prof Rajendra Prasad
School Of Life Sciences
J.N.U;NEW DELHI - 110067

--- On Sun, 3/7/11, yeast-request from oat.bio.indiana.edu <yeast-request from oat.bio.indiana.edu> wrote:


From: yeast-request from oat.bio.indiana.edu <yeast-request from oat.bio.indiana.edu>
Subject: Yeast Digest, Vol 68, Issue 1
To: yeast from magpie.bio.indiana.edu
Date: Sunday, 3 July, 2011, 10:33 PM


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Today's Topics:

   1. 5-FOA (kgeiler77)


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Message: 1
Date: Mon, 27 Jun 2011 10:53:26 -0700 (PDT)
From: kgeiler77 <kgeiler from fas.harvard.edu>
Subject: [Yeast] 5-FOA
To: yeast from magpie.bio.indiana.edu
Message-ID: <31939996.post from talk.nabble.com>
Content-Type: text/plain; charset=us-ascii


Hi,

I was wondering what false positive rate to expect when plating yeast on
5-FOA. Are these false positives due to Ura+ colonies that manage to live on
this media for stochastic reasons (and can be screened for by replica
plating onto 5-FOA a second time), or do they represent mutations that allow
survival of Ura+ colonies on 5-FOA?

Any help, even general comments on false positives and 5-FOA, would be
greatly appreciated.

Thanks,
KG
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