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--></style><title>Mating type switching in S. cerevisiae
Katrin Hartwich</title></head><body>
<div>Hello,</div>
<div><br></div>
<div>My former advisor, Jim Haber, provided SGD with some text that
explains the problem: see <a
href="http://db.yeastgenome.org/cgi-bin/locus.pl?locus=mat"
>http://db.yeastgenome.org/cgi-bin/locus.pl?locus=mat</a> then go to
"summary paragraph" <a
href=
"http://db.yeastgenome.org/cgi-bin/locus.pl?locus=mat#summaryParagraph"
>http://db.yeastgenome.org/cgi-bin/locus.pl?locus=mat#summaryParagraph</a
></div>
<div><br></div>
<div>Basically, MATa in the S288c background (BY4141 is S288c
background) has a single base pair polymorphism that greatly impairs
(but does not completely prevent) MAT switching.</div>
<div><br></div>
<div>Regards,</div>
<div><br></div>
<div>John McCusker</div>
<div><br></div>
<div>*****************************************</div>
<div><font face="Courier" size="-4" color="#000000">Hello to all,<br>
<br>
I'm working with the S. cerevisiae strains from the EUROSCARF
collection<br>
(BY4741). All strains have the mating type a and I want to switch now
the<br>
mating type of some of them to alpha. I have a protocol and a plasmid
for<br>
mating type switching from a friend but it didn't work that well.
Does<br>
anybody have a good working protocol/plasmid for mating type switching
for<br>
me?<br>
Thank's a lot!<br>
<br>
Best wishes,<br>
Katrin<br>
<br>
<br>
<br>
--<br>
Katrin Hartwich<br>
European Neuroscience Institute Göttingen (ENI-G)<br>
Molecular Neurogenetics<br>
<br>
Grisebachstraße 5<br>
37073 Göttingen<br>
Germany<br>
<br>
Phone: +49 (0)551-39-13972<br>
Fax: +49 (0)551-39-12346<br>
Email:</font><font face="Courier" size="-4" color="#551A8B"><u>
khartwi from gwdg.de</u></font><br>
<font face="Courier" size="-4" color="#000000"></font></div>
<x-sigsep><pre>--
</pre></x-sigsep>
<div>*************************************************<br>
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<br>
John H. McCusker, Assoc. Prof.<br>
Dept. of Molecular Genetics & Microbiology, 3020<br>
Duke University Medical Center<br>
Durham, NC 27710<br>
<br>
http://www.duke.edu/web/microlabs/mccusker/<br>
phone: (919) 681-6744<br>
fax: (919) 684-8735<br>
e-mail: mccus001@mc.duke.edu<br>
<br>
PLASMID REQUESTS: PLEASE CHECK WEB SITE</div>
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