GIL .. BIOSCI/Bionet News .. Biosequences .. Software .. FTP

A book that might be useful is:<br><br>Wolf, Bruenig, Barth (Eds.). 2003. Non-convential yeasts in genetics, biochemistry, and biotechnology. Springer.<br><br>There's nothing specifically on S. kluyveri but it does have a couple of protocols for doing gene disruptions in K. lactis which is known to exhibit low rates of homologous recombination. <br><br>Cheers,<br>Paul Magwene<br><br><br><div class="gmail_quote">On Dec 10, 2007 11:09 AM, Christian Landry <<a href="mailto:clandry@post.harvard.edu">clandry@post.harvard.edu</a>> wrote:<br><blockquote class="gmail_quote" style="border-left: 1px solid rgb(204, 204, 204); margin: 0pt 0pt 0pt 0.8ex; padding-left: 1ex;"> <div style="">Hello,<div><br></div><div>Does anyone know if it is feasible to do homologous recombination (with PCR products) of Saccharomyces kluyveri? Do you know any reference?</div><div>Finally,  do you know who would have haploid strains (a and alpha) for this species with selectable markers? </div><div><br></div><div>Thanks a lot,</div><font color="#888888"><div><br></div><div>Christian<br><br><div> <span style="border-collapse: separate; border-spacing: 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px;"> <span style="border-collapse: separate; border-spacing: 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px;"> <span style="border-collapse: separate; border-spacing: 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px;"> <span style="border-collapse: separate; border-spacing: 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px;"> <span style="border-collapse: separate; border-spacing: 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px;"> <span style="border-collapse: separate; border-spacing: 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px;"> <span style="border-collapse: separate; border-spacing: 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px;"> <div style="margin: 0px;"><br></div></span></span></span></span></span></span></span></div></div></font></div><br>_______________________________________________<br>Yeast mailing list<br><a href="mailto:Yeast@net.bio.net"> Yeast@net.bio.net</a><br><a href="http://www.bio.net/biomail/listinfo/yeast" target="_blank">http://www.bio.net/biomail/listinfo/yeast</a><br></blockquote></div><br>

Send comments to us at archive@iubio.bio.indiana.edu